Investigation of the Effectiveness of Quercetin on Acrosome Integrity of Merino Ram Sperm

To achieving high efficiency in sheep, ram sperm must have fertilization ability. Quercetin, a potent antioxidant, has been shown to positive effect on semen different species. This study aims improve the of Merino using quercetin. The ejaculates collected from rams were used for dilution (sperm density ≥ 3×109 spermatozoa/ml, motility 75%). Tris diluent was dilute semen. divided into 4 equal volumes at 32 °C. Three groups formed by adding quercetin (15, 30, 45 μg/mL) doses. Antioxidant (quercetin) did not participate 4th group (the control group). Spermatozoa diluted and frozen approximately 4x108 spermatozoa per ml with diluent. After thawing, master mix prepared 60μl semen, 10μl FITC-PNA 2.5μl PI allowed incubate 37 °C 20 minutes. reaction then terminated after treatment 10μL Hancock solution. An amount sample taken 200 counted 400 magnifications fluorescent microscope. Sperm cells green color evaluated as damaged acrosome, no colour regarded undamaged acrosome. In terms percentage acrosomal integrity assessed 45μg/mL). Findings: 39.16 ± 2.65b 15μg/mL group, 40.83 0.30b 30μ/mL 20.83 2.95a 45μg/mL 54.83 3.38c group. three substance added doses 45μg/mL) lower than Quercetin toxicize acrosome these